ESI and nanoESI - CID - MS / MS
نویسندگان
چکیده
منابع مشابه
Folded emitters for nanoelectrospray ionization mass spectrometry.
Electrospray ionization (ESI) has revolutionized mass spectrometry (MS), providing a facile method for the ionization of macromolecules for analysis by mass. The development of nanoESI-MS has further extended the utility of ESI-MS, permitting the analysis of small-volume samples with enhanced sensitivity over conventional ESI-MS. Traditional nanoESI-MS experiments use pulled-glass capillary emi...
متن کاملStructural Analysis of -Carrageenan Sulfated Oligosaccharides by Positive Mode Nano-ESI- FTICR-MS and MS/MS by SORI-CID
Structural analysis of sulfated oligosaccharides from -carrageenan of up to ten residues (MW 2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the Band Y-types of fragmentation were observed and enabled complete sequencing of the ol...
متن کاملDetermination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry.
Chondroitin sulfate (CS) and dermatan sulfate (DS) glycosaminoglycans display variability of sulfation in their constituent disaccharide repeats during chain elongation. Since a large proportion of the extracellular matrix of the central nervous system (CNS) is composed of proteoglycans, CS/DS disaccharide degree and profile of sulfation play important roles in the functional diversity of neuro...
متن کاملDynamic Variation Patterns of Aconitum Alkaloids in Daughter Root of Aconitum Carmichaelii (Fuzi) in the Decoction Process Based on the Content Changes of Nine Aconitum Alkaloids by HPLC- MS- MS
The chemical components in the decoctions of Chinese herbal medicines are not always the same as those in the crude herbs because of the insolubility or instability of some compounds. In this work, a high-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry method was developed to explore dynamic variation patterns of aconitum alkaloids in...
متن کاملThe Peptide MS/MS-Fragmentome: A Set of Predictable Fragment Ions with Highly Redundant Sequence Information
Extended Abstract Low-energy collision-induced dissociation (CID) of peptides in a collision cell has been introduced as a fast method for peptide sequencing [1]. The method has been adapted to the analysis of gel-isolated proteins by the introduction of the nanoESI technology [2]. In the meantime proteins can be identified by search-engine supported interpretation of raw data of peptide MS/MS ...
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